Difference between revisions of "Part:BBa K1918102:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
AIM-the expression of EBFP2C(155-238) would be induced by Dox.
+
ligation from [pwx226(EcoRI&PspOMI)][pwx161(WL16056&WL16052)]
  
 
===Source===
 
===Source===

Revision as of 07:58, 14 October 2016


TRE-Kozak-EBFP2C(155-238)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 649
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 649
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ligation from [pwx226(EcoRI&PspOMI)][pwx161(WL16056&WL16052)]

Source

[pwx226(EcoRI&PspOMI)]
[pwx161(WL16056&WL16052)]

References