Difference between revisions of "Part:BBa K2066037"
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<partinfo>BBa_K2066037 short</partinfo>
 
<partinfo>BBa_K2066037 short</partinfo>
  
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<p>This part is part of William and Mary iGEM 2016's library of IPTG-inducible RBS characterization parts. It contains the RBS BBa_B0031. </p>
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to be completed
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<p>The part codes for the expression of a superfolder GFP and is regulated by a lacI-repressible plLacO-1 Promoter. By adding IPTG one should be able to induce the expression of sfGFP and compare the induction curve to other IPTG-inducible RBS characterization parts from our library to determine the relative strengths of different RBS sequences across an induction curve.</p>
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<p>We have included the self-cleaving ribozyme RiboJ immediately upstream of the RBS sequence in order to buffer against translational influence from the 5' untranslated region conferred to the transcript by the promoter sequence.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:07, 14 October 2016


pACT-Tet on UNS Standard

to be completed

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 969
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]