Difference between revisions of "Part:BBa K2089005"

Latest revision as of 03:57, 14 October 2016

HHL1 protein from Arabidopsis

Gene hhl1 encodes protein HHL1,which is produced and distributed among chloroplasts(especailly grana core-,grana margin and stroma lamellae enriched thylokoid membranes) to function in protecting and repairing photosystemⅡfrom photodamage. Gene hhl1 encode a 231-amino acids protein HHL1.HHL1 helps protect the PSⅡagainst photodamage and help increase quantum yield.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Gene hhl1 encode a 231-amino acids protein HHL1.HHL1 helps protect the PS&#8545;against photodamage and help increase quantum yield.
-In order to examine the efficiency of this device,we made a part which consists of a complete functional protein HHL1 coding sequence in Arabidopsis and gene hhl1,and this part has been taken into the nucleus of protoplasts of Arabiodopsis using psb1c3 plasmid as its carrier.RT-PCR is done to the transcribed mRNA of gene hhl1 to get cDNA,then we use the ultraviolet spectrophotometer to measure the concentration of cDNA we got.The concentration data is used to calculate the relative expression level of gene hhl1 compared to the gene expression of gene hhl1 regulated by the promoter pif1,cop1 and phyB. From the result,this part has no significent change in expression level of mRNA in growth light(100lux) and in high light(1200lux).This means that the promoter pHhl1 is repressed in these light intensities so the expression level is quite low.
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