Difference between revisions of "Part:BBa K1997021"
(No difference)
|
Revision as of 01:20, 14 October 2016
P+R->sLuc-N->Zif268->sLuc-C->TER
This part is an integrated tool for protein-protein interaction research using split-LUC system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1777
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1780
Illegal BsaI.rc site found at 1513