Difference between revisions of "Part:BBa K1997012"
(No difference)

Revision as of 00:08, 14 October 2016


P+R->sHRP-N->Zif268-> sHRP-C->TER

This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1171
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1174
    Illegal BsaI.rc site found at 907
    Illegal SapI.rc site found at 1288