Difference between revisions of "Part:BBa K2020050"

Revision as of 23:07, 13 October 2016

wild type Mj Y-Synthetase for use in E.coli

This is the wild type tyrosyl synthetase derived from Methanococcus janaschii to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate Tyrosin in response to an amber stop codon.

Usage and Biology

Control for incorporation of ncAA

Assembly in a synthetase plasmid for incorporation of ncAA

pACYC derived plasmid with Mj tyrosyl synthetase and cognate tRNA

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 84
Illegal SapI.rc site found at 877

Revision as of 23:06, 13 October 2016 (view source) Andrea hoeltken (Talk \| contribs) ← Older edit		Revision as of 23:07, 13 October 2016 (view source) Andrea hoeltken (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2020050 short</partinfo>		<partinfo>BBa_K2020050 short</partinfo>

−	This is the wild type tyrosyl synthetase derived from Methanococcus janaschii to be used as a orthogonal synthetase in E.coli. This part can be used together with the [~~https://parts.igem.org/~~Part:BBa_K2020042 cognate tRNA BBa_K2020042] to incorporate Tyrosin in response to an amber stop codon.	+	This is the wild type tyrosyl synthetase derived from Methanococcus janaschii to be used as a orthogonal synthetase in E.coli. This part can be used together with the [[Part:BBa_K2020042\|cognate tRNA BBa_K2020042]] to incorporate Tyrosin in response to an amber stop codon.