Difference between revisions of "Part:BBa K2020050"
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====Assembly in a synthetase plasmid for incorporation of ncAA====
 
====Assembly in a synthetase plasmid for incorporation of ncAA====
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[[File:T--Aachen--Mj YRS CUA.jpg|200px|thumb|left|pACYC derived plasmid with Mj tyrosyl synthetase and cognate tRNA]]
  
  

Revision as of 23:05, 13 October 2016


wild type Mj Y-Synthetase for use in E.coli

This is the wild type tyrosyl synthetase derived from Methanococcus janaschii to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate Tyrosin in response to an amber stop codon.


Usage and Biology

Control for incorporation of ncAA

Assembly in a synthetase plasmid for incorporation of ncAA

pACYC derived plasmid with Mj tyrosyl synthetase and cognate tRNA


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 84
    Illegal SapI.rc site found at 877