Difference between revisions of "Part:BBa K2020042:Experience"

Revision as of 21:23, 13 October 2016

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2020042

[http://2014.igem.org/Team:Austin_Texas/kit iGEM Team Austin, Texas 2016 - Expanded Genetic Code Measurement Kit]

The tRNA is used together with a tRNA-Synthetase. It has been proven to work with (enter links for parts)

Y-RS
oNBY-RS
AzF
CN-F synthetase
Iodo-Y synthetase
5HT-P synthetase
Nitro-Y synthetase
Amino-Y synthetase

[http://2014.igem.org/Team:Austin_Texas/photocage iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase]

iGEM Team Austin, Texas 2014 insorporated oNBY into T7 RNA polymerase.

[http://2016.igem.org/Team:Aachen iGEM-Team Aachen 2016]

[http://2016.igem.org/Team:Aachen/Description LIPS - Light inducible proteases]

iGEM Team Aachen 2016 used the tRNA to successfully incorporate tyrosine, oNB-Y and DMNB-S in E.coli BL21 DE3 gold with their newly designed DMNBS-RS

User Reviews

UNIQ7446a309897602f0-partinfo-00000000-QINU UNIQ7446a309897602f0-partinfo-00000001-QINU

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.

[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.

@@ Line 17: / Line 17: @@
 ====[http://2014.igem.org/Team:Austin_Texas/photocage iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase]====
+iGEM Team Austin, Texas 2014 insorporated oNBY into T7 RNA polymerase.
 ====[http://2016.igem.org/Team:Aachen iGEM-Team Aachen 2016]====