Difference between revisions of "Part:BBa K1968002"
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RBS* is a ribosome binding site that has been specifically designed to include the anti-Shine Dalgarno (SD) sequence of Synechocystis sp. strain PCC 6803. If no extra bases are added to its sequence, RBS* has an optimal spacing of 9bp between the central base of the core SD sequence (which is base A at position 7 of its sequence) and the first base of the start codon (1). RBS* has been shown to be 5 times stronger than BBa_B0034 in Synechocystis (1), making it the strongest currently available RBS for heterologous gene expression in this cyanobacterium. In E. coli, RBS* is one-third weaker than BBa_B0034. | RBS* is a ribosome binding site that has been specifically designed to include the anti-Shine Dalgarno (SD) sequence of Synechocystis sp. strain PCC 6803. If no extra bases are added to its sequence, RBS* has an optimal spacing of 9bp between the central base of the core SD sequence (which is base A at position 7 of its sequence) and the first base of the start codon (1). RBS* has been shown to be 5 times stronger than BBa_B0034 in Synechocystis (1), making it the strongest currently available RBS for heterologous gene expression in this cyanobacterium. In E. coli, RBS* is one-third weaker than BBa_B0034. | ||
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(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X | (1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X |
Latest revision as of 21:09, 13 October 2016
Synechocystis RBS* Phytobrick
RBS* is a ribosome binding site that has been specifically designed to include the anti-Shine Dalgarno (SD) sequence of Synechocystis sp. strain PCC 6803. If no extra bases are added to its sequence, RBS* has an optimal spacing of 9bp between the central base of the core SD sequence (which is base A at position 7 of its sequence) and the first base of the start codon (1). RBS* has been shown to be 5 times stronger than BBa_B0034 in Synechocystis (1), making it the strongest currently available RBS for heterologous gene expression in this cyanobacterium. In E. coli, RBS* is one-third weaker than BBa_B0034.
(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]