Difference between revisions of "Part:BBa K2148013:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The Gly-Ser linker is advised by Dr N. Patron (Earlham Institute)
+
Two Guanine nucleotides added to downstream of the CDS to create a Gly-Ser linker is advised by Dr N. Patron (Earlham Institute), which allows for the creation of fusion proteins. The stop codon is also removed for this purpose. Multiple Bpe1 sites within the CDS was removed without changing the amino acid it translates to.
  
  

Latest revision as of 20:21, 13 October 2016


Cas9 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two Guanine nucleotides added to downstream of the CDS to create a Gly-Ser linker is advised by Dr N. Patron (Earlham Institute), which allows for the creation of fusion proteins. The stop codon is also removed for this purpose. Multiple Bpe1 sites within the CDS was removed without changing the amino acid it translates to.


Source

Streptococcus pyogenes

References