Difference between revisions of "Part:BBa K2120312"
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J23119、J23116、J23109、J23106 are all constitutive promoter，tetR is an inhibitor which is built to repress the expression of Ptet promoter. The expression efficiency of tetR depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor. | J23119、J23116、J23109、J23106 are all constitutive promoter，tetR is an inhibitor which is built to repress the expression of Ptet promoter. The expression efficiency of tetR depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor. | ||
Revision as of 19:55, 13 October 2016
J23119+B0034+tetR+B0015
J23119、J23116、J23109、J23106 are all constitutive promoter，tetR is an inhibitor which is built to repress the expression of Ptet promoter. The expression efficiency of tetR depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]