Difference between revisions of "Part:BBa K2120309"

 
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<partinfo>BBa_K2120309 short</partinfo>
 
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pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And LacIq is an inhibitor which is built to repress the expression of PLac promoter. We use it to control the “on” or ”off” state of toxin gene.YFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120304 】.
  
 
    
 
    

Revision as of 18:49, 13 October 2016


B0015+CI+B0034+pBAD-AraC+pR+B0032+RFP+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And LacIq is an inhibitor which is built to repress the expression of PLac promoter. We use it to control the “on” or ”off” state of toxin gene.YFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120304 】.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1166
    Illegal AgeI site found at 2788
    Illegal AgeI site found at 2900
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1183