Difference between revisions of "Part:BBa K1937005:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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+ | The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937006).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag. | ||
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+ | [[File:BBa K1937003-results.png]] | ||
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+ | We indeed observed some red stains on the colonies only with the pNagP promoter growing on NAG (as well as with the pNagA promoter, see part BAA_XXXXX), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose). | ||
===Applications of BBa_K1937005=== | ===Applications of BBa_K1937005=== |
Latest revision as of 17:46, 13 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937006).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag.
We indeed observed some red stains on the colonies only with the pNagP promoter growing on NAG (as well as with the pNagA promoter, see part BAA_XXXXX), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose).
Applications of BBa_K1937005
User Reviews
UNIQb2badcf878e4a6b9-partinfo-00000000-QINU UNIQb2badcf878e4a6b9-partinfo-00000001-QINU