Difference between revisions of "Part:BBa K2110107"
 
Line 9: Line 9:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K2110107 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2110107 SequenceAndFeatures</partinfo>
−
Activity Assay<br/>
+
===Results===
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
−
https://static.igem.org/mediawiki/parts/2/27/T--Tianjin--partL117F.png
+
https://static.igem.org/mediawiki/parts/thumb/7/79/T--Tianjin--partdataL117F.png/800px-T--Tianjin--partdataL117F.png
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 17:32, 13 October 2016


PETase site-directed mutant L117F

This is one of the site-directed mutant of the PETase gene, we change the 117th amino acid from L to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BglII site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. 800px-T--Tianjin--partdataL117F.png