Difference between revisions of "Part:BBa K1935009:Design"
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Latest revision as of 13:31, 13 October 2016
pMYO-3 : Specific muscle promoter of C.elegans
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 53
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1446
Design Notes
Mutagenesis was done to abolish three illegal restriction sites : SpeI (1018-1023), PstI (1212-1217) and XbaI (2025-2030).
Source
PROMOTEROME : http://worfdb.dfci.harvard.edu/promoteromedb/searchPromoterome.pl?by=name&sid=myo-3
References
http://www.wormbase.org/species/c_elegans/gene/WBGene00003515#01-9g-3