Difference between revisions of "Part:BBa K2110109"
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<partinfo>BBa_K2110109 short</partinfo>
 
<partinfo>BBa_K2110109 short</partinfo>
  
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This is one of the site-directed mutant of the PETase gene, we change the 238th amino acid from A to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
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This is one of the site-directed mutant of the PETase gene, we change the 238th amino acid from S to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
  
 
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Revision as of 13:05, 13 October 2016


PETase site-directed mutant S238F

This is one of the site-directed mutant of the PETase gene, we change the 238th amino acid from S to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BglII site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Activity Assay
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. T--Tianjin--partA238F.png