Difference between revisions of "Part:BBa K1937003:Design"
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+ | The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). | ||
+ | The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping. | ||
+ | [[File:BBa_K1937003_-construction.png]] |
Revision as of 12:43, 13 October 2016
The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.