Difference between revisions of "Part:BBa K1937003:Experience"
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===Applications of BBa_K1937003=== | ===Applications of BBa_K1937003=== | ||
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| + | The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937004).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag. | ||
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| + | [[File:BBa_K1937003-results.png]] | ||
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| + | We indeed observed some red stains on the colonies only with the pNagA promoter growing on NAG (as well as with the pNagP promoter, see part BBa_K1937006), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose) | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 12:40, 13 October 2016
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1937003
The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937004).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag.
We indeed observed some red stains on the colonies only with the pNagA promoter growing on NAG (as well as with the pNagP promoter, see part BBa_K1937006), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose)
User Reviews
UNIQ069411f7348c8186-partinfo-00000000-QINU UNIQ069411f7348c8186-partinfo-00000001-QINU