Difference between revisions of "Part:BBa K2110105"
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| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K2110105 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2110105 SequenceAndFeatures</partinfo> | ||
| − | Activity Assay<br/> | + | ===Activity Assay===<br/> |
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. | We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. | ||
| − | https://static.igem.org/mediawiki/parts/7/70/T--Tianjin--partS207T.png | + | <p style="text-align:center">https://static.igem.org/mediawiki/parts/7/70/T--Tianjin--partS207T.png</p> |
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Revision as of 09:17, 13 October 2016
PETase site-directed mutant I208V
This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
Illegal BglII site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
===Activity Assay===
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
