Difference between revisions of "Part:BBa K1613017"

Revision as of 08:05, 13 October 2016

K1613017 is a new part which can detect lead (Pb) ions in the liquid.

This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 with a constitutive promoter J23102 and RFP to make them function.

Whether lead can enter e.coli or not: By using gold nanoparticles(AU-NPs), we measured the lead absorption of e.coli. The results showed that the longer e.coli put in Pb2+ or the higher Pb2+ concentration, the more lead entered the e.coli.

iGEM16_HSiTAIWAN: Measure fluorescence of lead detector File:HSiTAIWAN BACKGROUND PROJECT Pb Figure 7 Fluorescence of Lead Biosensor.jpeg

Sequence and Features