Difference between revisions of "Part:BBa K1499004:Experience"

Revision as of 07:41, 13 October 2016

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1499004

Group: 2016 Stanford-Brown iGEM Team Author: Michael Becich Summary: The 2016 Stanford-Brown iGEM Team purified this linker protein and used it to create a BioDevice. Used in tandem with a biotinylated fluorophore, this CBD/Streptavidin fusion protein served as a linker between cellulose paper and the fluorophore-quencher biosensor described here: http://2016.igem.org/Team:Stanford-Brown/SB16_BioSensor_FQsensor. Uploads: (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.)

User Reviews

UNIQ4098530d6dcfee87-partinfo-00000000-QINU

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mbecich

The part worked as specified for our needs! It should be noted that an inducible promoter, HisTag, and double terminator are all included in this part to facilitate expression and purification. This was confirmed to us by the part creator and former Stanford-Brown iGEMer herself, Alaina Shumate.

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