Difference between revisions of "Part:BBa K2150023"

Latest revision as of 03:51, 13 October 2016

pCon+RBS+tetR+DT+pTet+RBS+T7RNP+Ter+RBS+pT7+GFP+DT

This part is a complete regulatory device sensitive to tetracycline. The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no T7RNAP is produced, resulting in the inactivity of pT7. Thus, no GFP is expressed and no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of T7RNAP and activation of pT7 which further leads to the expression and amplification of GFP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. This part is used to test the tet switch and the tet-on/tet-off system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4379

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2150023 short</partinfo>
-This part is a complete regulatory device sensitive to tetracycline. The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no T7RNAP is produced, resulting in the inactivity of pT7. Thus, no GFP is expressed and no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of T7RNAP and activation of pT7 which further leads to the expression and amplification of GFP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. This part is used to test the tet switch and the tet-on/tet-off system.
+This part is a complete regulatory device sensitive to tetracycline. The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no T7RNAP is produced, resulting in the inactivity of pT7. Thus, no GFP is expressed and no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of T7RNAP and activation of pT7 which further leads to the expression and amplification of GFP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. This part is used to test the tet switch and the tet-on/tet-off system.
 <!-- Add more about the biology of this part here