Difference between revisions of "Part:BBa K2042014"

Revision as of 22:00, 12 October 2016

RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)

This is the sequence of the PLA producing operon. This sequence is optimized for P.putida and is constituted of two gene propionate CoA transferase (Pct) and PHA synthase (PhaC). Pct contain a mutation that allows him to take lactate as the acceptor and convert it efficiently into lactyl-CoA. The PhaC gene contains 4 amino acid mutation which were found to polymerize monomer of lactyl-coA into PLA homopolymer. This gene is placed upstream in the sequence closer of the promoter because polymerization is the bottleneck of the PLA producing system.