Difference between revisions of "Part:BBa K2066030"
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<partinfo>BBa_K2066030 short</partinfo>
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pTAC Ribozyme Characterization Part – GFP only; no RiboJ
 
pTAC Ribozyme Characterization Part – GFP only; no RiboJ
  
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This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
 
This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2066030 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2066030 parameters</partinfo>
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Revision as of 18:46, 12 October 2016

pTAC sfGFP

pTAC Ribozyme Characterization Part – GFP only; no RiboJ


This part was designed to be assembled via Gibson Assembly from WM16_014 (using primers WM16_P027 and WM16_P009) and the same WM16_014 again (using primers WM16_P019 and WM16_P025).

This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 132