Difference between revisions of "Part:BBa K2042004"

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For this experiment the cell grew in the 30ºC chamber with shaking until they reached OD of around 0.4 (spechtophotometre indicated that the OD was 0.4, the TECAN in which the fluorescence was read says it is 0.2 in the beginning, but this OD is irrelevant since the values only matter for normalization).
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When the OD was reached, induction with the indicated concentrations of IPTG was made, the culture was putted back in the 30'C chamber with shaking. Timepoints was taken and measured directly in the TECAN.
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These are the results.
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Values for graph without correction of OD600
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Revision as of 16:06, 12 October 2016


IPTG inducible promoter

This is the sequence of an IPTG inductible promoter working in Pseudomonas putida.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

For this experiment the cell grew in the 30ºC chamber with shaking until they reached OD of around 0.4 (spechtophotometre indicated that the OD was 0.4, the TECAN in which the fluorescence was read says 0.2 in the beginning, but this OD is irrelevant since the values only matter for normalization). When the OD was reached, induction with the indicated concentrations of IPTG was made, the culture was put into a 96-wells plate, and measured on the TECAN for 1hour. (Note, the measure was made without shaking) These are the results that the TECAN automatically provides.


For this experiment the cell grew in the 30ºC chamber with shaking until they reached OD of around 0.4 (spechtophotometre indicated that the OD was 0.4, the TECAN in which the fluorescence was read says it is 0.2 in the beginning, but this OD is irrelevant since the values only matter for normalization). When the OD was reached, induction with the indicated concentrations of IPTG was made, the culture was putted back in the 30'C chamber with shaking. Timepoints was taken and measured directly in the TECAN. These are the results. Values for graph without correction of OD600