Difference between revisions of "Part:BBa K2150016"
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<partinfo>BBa_K2150015 short</partinfo>
 
<partinfo>BBa_K2150015 short</partinfo>
  
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T7 promoter and RBS and GFP, with a double terminator. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.
+
T7 promoter and RBS and GFP, with double terminators. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:54, 12 October 2016


pTet TetX-GFP(fusion protein)

T7 promoter and RBS and GFP, with double terminators. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1939