Difference between revisions of "Part:BBa K1884001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA bindin domian fused to its N terminus | + | This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA bindin domian fused to its N terminus. In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor. |
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Revision as of 08:48, 12 October 2016
Gal4BD-CIB1
Cryptochrome-interacting basic-helix-loop-helix 1(CIB1) is a protein-coded gene. The product of this gene expression is a basic helix-loop-helix (bHLH) protein which would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to N terminus of CIB1
Usage and Biology
This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA bindin domian fused to its N terminus. In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 218
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 637
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 137