Difference between revisions of "Part:BBa K2089004"

 
 
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pHhl1 is the promoter of gene hhl1 in wild type Arabidopsis,the promoter may be regulated by the concentration of certain protein differs from before and after photodamage takes place in photosystemⅡ.  
 
pHhl1 is the promoter of gene hhl1 in wild type Arabidopsis,the promoter may be regulated by the concentration of certain protein differs from before and after photodamage takes place in photosystemⅡ.  
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In order to examine the efficiency of this promoter,we made a part which consists of a complete functional protein HHL1 coding sequence in Arabidopsis and gene hhl1,and this part has been taken into the nucleus of protoplasts of Arabiodopsis using psb1c3 plasmid as its carrier.RT-PCR is done to the transcribed mRNA of gene hhl1 to get cDNA,then we use the ultraviolet spectrophotometer to measure the concentration of cDNA we got.The concentration data is used to calculate the relative expression level of gene hhl1 compared to the gene expression of gene hhl1 regulated by the promoter pif1,cop1 and phyB.
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From the result,this part has no significent change in expression level of mRNA in growth light(100lux) and in high light(1200lux).This means that the promoter pHhl1 is repressed in these light intensities so the expression level is quite low.
  
 
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===Usage and Biology===
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Latest revision as of 05:10, 12 October 2016


pHHL from Arabidopsis

pHhl1 is the promoter of gene hhl1 in wild type Arabidopsis,the promoter may be regulated by the concentration of certain protein differs from before and after photodamage takes place in photosystemⅡ.

In order to examine the efficiency of this promoter,we made a part which consists of a complete functional protein HHL1 coding sequence in Arabidopsis and gene hhl1,and this part has been taken into the nucleus of protoplasts of Arabiodopsis using psb1c3 plasmid as its carrier.RT-PCR is done to the transcribed mRNA of gene hhl1 to get cDNA,then we use the ultraviolet spectrophotometer to measure the concentration of cDNA we got.The concentration data is used to calculate the relative expression level of gene hhl1 compared to the gene expression of gene hhl1 regulated by the promoter pif1,cop1 and phyB. From the result,this part has no significent change in expression level of mRNA in growth light(100lux) and in high light(1200lux).This means that the promoter pHhl1 is repressed in these light intensities so the expression level is quite low.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 456
    Illegal SpeI site found at 1469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal SpeI site found at 456
    Illegal SpeI site found at 1469
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 456
    Illegal SpeI site found at 1469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 456
    Illegal SpeI site found at 1469
    Illegal NgoMIV site found at 706
  • 1000
    COMPATIBLE WITH RFC[1000]