Difference between revisions of "Part:BBa K1897027"
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Revision as of 16:23, 11 October 2016
J23100-33-ALR-Terminator
The construct contains a high expression promoter J23100 in a cassette (BBa_K314100) with a weaker RBS (BBa_B0033), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493
Illegal NheI site found at 854 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 778
Illegal BamHI site found at 1480 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
Illegal AgeI site found at 896
Illegal AgeI site found at 1196 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 653