Difference between revisions of "Part:BBa K1897025"

Revision as of 16:22, 11 October 2016

J23100-34-ALR-Terminator

The construct contains a high expression promoter J23100 in a cassette (BBa_K314100) with a strong RBS (BBa_B0034), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 470
Illegal NheI site found at 493
Illegal NheI site found at 855
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 779
Illegal BamHI site found at 1481
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 126
Illegal AgeI site found at 897
Illegal AgeI site found at 1197
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 654

Revision as of 16:20, 11 October 2016 (view source) Registry (Talk \| contribs)		Revision as of 16:22, 11 October 2016 (view source) Keshiniy (Talk \| contribs) m Newer edit →
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	<partinfo>BBa_K1897025 short</partinfo>		<partinfo>BBa_K1897025 short</partinfo>

−	The construct contains a high expression promoter J23100 with a strong RBS (BBa_B0034~~) in a cassette (BBa_K314100~~), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator.	+	The construct contains a high expression promoter J23100 in a cassette (BBa_K314100) with a strong RBS (BBa_B0034), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator.