Difference between revisions of "Part:BBa K1897012:Design"

 
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===Source===
 
===Source===
  
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Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is _______
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Listeriolysin O (LLO) is derived from <i>Listeria monocytogenes</i> and is initially obtained from <partinfo>BBa_K177026</partinfo>. However, after sequencing, the results obtained for sequencing of <partinfo>BBa_K177026</partinfo> did not appear to match that given on the page. We went on to synthesize new LLO (for <partinfo> BBa_K1897013</partinfo>) with the results obtained from sequencing.
  
 
===References===
 
===References===

Revision as of 15:12, 11 October 2016


Listeriolysin O CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1380
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimised for expression in Escherichia coli.


Source

Listeriolysin O (LLO) is derived from Listeria monocytogenes and is initially obtained from BBa_K177026. However, after sequencing, the results obtained for sequencing of BBa_K177026 did not appear to match that given on the page. We went on to synthesize new LLO (for No part name specified with partinfo tag.) with the results obtained from sequencing.

References