Difference between revisions of "Part:BBa K1920001"
 
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<partinfo>BBa_K1920001 short</partinfo>
 
<partinfo>BBa_K1920001 short</partinfo>
  
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Glucose detection device , which uses red fluroscence protein E1010 as reportor .
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Glucose detection device, which uses red fluorescence protein E1010 as a Reporter . PI promoter consists of overlapping consensus CRP-binding sites and consensus RNA polymerase binding site. Therefore, the steric hindrance between CRP and RNA polymerase will cause downstream genes to be repressed at a high concentration of CRP. In E.coli strains( e.g.DH5α and Bl21), a high concentration of CRP is presented when the glucose concentration is low, because, with the increasing adenylyl cyclase activity, the cAMP increases. cAMP will bind the cAMP receptor protein (CRP) which, in its bound form, can specifically bind the consensus CRP-binding site of Pl promoter , leading to repression of downstream genes. In contrast, when the glucose concentration is high, the adenylyl cyclase activity, cAMP, and CRP are decreased in the bound form . Consequently, an increased expression of downstream RFP E1010 can be a reporter gene as well as an indicator of the presence of glucose can be.
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PI promoter consists of overlaping consensus CRP-binding site and consensus RNA polymerase binding site. Therefore,the steric hindrance between CRP and RNA polymerase will cause downstream gene to be repressed at high concentration of CRP.
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In E.coli strains,e.g.DH5α and Bl21,high concentration of CRP is present when glucose concentration is low ,resulting in increased adenylyl cyclase activity and increased cAMP . cAMP will bind the cAMP receptor protein (CRP) and CRP, in its bound form, will specifically bind the consensus CRP-binding site of Pl promoter , resulting in downstream gene repression.
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This part works and is experimentally validated by NCKU_Tainan_2016 , further details are documented in experience page.
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In contrast , if glucose concentration is high , there will be decreased adenylyl cyclase activity ,decreased cAMP ,and decreased CRP in bound form , resulting in increased expression of downstream RFP E1010 as a reporter gene as indicator of the presence of glucose.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 12:52, 11 October 2016


Pl-RBS-RFP-TT

Glucose detection device, which uses red fluorescence protein E1010 as a Reporter . PI promoter consists of overlapping consensus CRP-binding sites and consensus RNA polymerase binding site. Therefore, the steric hindrance between CRP and RNA polymerase will cause downstream genes to be repressed at a high concentration of CRP. In E.coli strains( e.g.DH5α and Bl21), a high concentration of CRP is presented when the glucose concentration is low, because, with the increasing adenylyl cyclase activity, the cAMP increases. cAMP will bind the cAMP receptor protein (CRP) which, in its bound form, can specifically bind the consensus CRP-binding site of Pl promoter , leading to repression of downstream genes. In contrast, when the glucose concentration is high, the adenylyl cyclase activity, cAMP, and CRP are decreased in the bound form . Consequently, an increased expression of downstream RFP E1010 can be a reporter gene as well as an indicator of the presence of glucose can be.

This part works and is experimentally validated by NCKU_Tainan_2016 , further details are documented in experience page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 617
    Illegal AgeI site found at 729
  • 1000
    COMPATIBLE WITH RFC[1000]