Difference between revisions of "Part:BBa K2114001"
(→‎Usage and Biology)
Line 8: Line 8:
 
===Usage and Biology===
 
===Usage and Biology===
  
 +
This part includes the anti-GFP nanobody (describted by Kubala et al. [Ref]) fused by an alpha helical linker [Ref] to the B. subtilis spore crust protein CotZ in order to be displayed on the spore surface. The hemagglutinin epitope tag was included in the fusion construct for convenient detection by specific anti-HA antibodies.
  
 +
[Figure of construct]
  
 +
===Characterization===
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:28, 11 October 2016


aGFPnano_HA_aHelix_cotZ

N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ.


Usage and Biology

This part includes the anti-GFP nanobody (describted by Kubala et al. [Ref]) fused by an alpha helical linker [Ref] to the B. subtilis spore crust protein CotZ in order to be displayed on the spore surface. The hemagglutinin epitope tag was included in the fusion construct for convenient detection by specific anti-HA antibodies.

[Figure of construct]

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 465
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]