Difference between revisions of "Part:BBa K1926011:Design"

 
Line 1: Line 1:
 +
_NOTOC__
 +
<partinfo>BBa_K1926001 short</partinfo>
  
 +
<partinfo>BBa_K1926001 SequenceAndFeatures</partinfo>
 +
 +
 +
===Design Notes===
 +
We got this sequence from human genome through PCR using the following primers:
 +
 +
pCCNE-F: CGTGTTTACATTCCACCCGCGCCA
 +
 +
pCCNE-R: TGATGGGGCTGCTCCGGCCT
 +
 +
(Product: 986)
 +
 +
===Source===
 +
 +
The sequence was retrieved from Addgene.

Revision as of 08:21, 11 October 2016

_NOTOC__ A cyclic promoter of Cyclin E from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 382
    Illegal NotI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 529
    Illegal XhoI site found at 1050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 273
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 491
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got this sequence from human genome through PCR using the following primers:

pCCNE-F: CGTGTTTACATTCCACCCGCGCCA

pCCNE-R: TGATGGGGCTGCTCCGGCCT

(Product: 986)

Source

The sequence was retrieved from Addgene.