Difference between revisions of "Part:BBa K1916100:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
From cyanobacteria genome
+
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
  
 
===References===
 
===References===

Revision as of 07:39, 11 October 2016


Ara-Inducible NpF2164g5 Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal SpeI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 131
    Illegal SpeI site found at 686
    Illegal NgoMIV site found at 1182
    Illegal AgeI site found at 1272
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Must be purified through chitin column.


Source

Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

References