Difference between revisions of "Part:BBa K1926003:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
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The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:
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The sequence was retrieved from Addgene.
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pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC
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−
pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA
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−
(Product: 457)
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−
 
+
  
 
===References===
 
===References===

Revision as of 04:16, 11 October 2016


A cyclic promoter of CDK4 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 522
    Illegal NheI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
    Illegal AgeI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got the sequence from human genome through PCR using the following primers:

pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC

pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA

(Product: 457)

Source

The sequence was retrieved from Addgene.

References