Difference between revisions of "Part:BBa K1916001"

 
 
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<partinfo>BBa_K1916001 short</partinfo>
 
<partinfo>BBa_K1916001 short</partinfo>
  
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A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with all CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light. RFC10 compatible.
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A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light. RFC10 compatible.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:43, 11 October 2016


NpF2164g5 + intein-CBD tag

A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light. RFC10 compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1027
    Illegal AgeI site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]