Difference between revisions of "Part:BBa K1926001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | We got this sequence from human genome through PCR using the following primers: | |
+ | pCCNE-F: CGTGTTTACATTCCACCCGCGCCA | ||
+ | pCCNE-R: TGATGGGGCTGCTCCGGCCT | ||
+ | |||
+ | (Product: 986) | ||
===Source=== | ===Source=== |
Revision as of 02:53, 11 October 2016
A cyclic promoter of Cyclin E from human genome
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 382
Illegal NotI site found at 497 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 529
Illegal XhoI site found at 1050 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 273
Illegal NgoMIV site found at 313
Illegal NgoMIV site found at 491 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We got this sequence from human genome through PCR using the following primers:
pCCNE-F: CGTGTTTACATTCCACCCGCGCCA
pCCNE-R: TGATGGGGCTGCTCCGGCCT
(Product: 986)
Source
The sequence was retrieved from Addgene.