Difference between revisions of "Part:BBa K1926001:Design"
(→Source) |
|||
| Line 14: | Line 14: | ||
The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: | The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: | ||
| + | |||
pCCNE-F: CGTGTTTACATTCCACCCGCGCCA | pCCNE-F: CGTGTTTACATTCCACCCGCGCCA | ||
| + | |||
pCCNE-R: TGATGGGGCTGCTCCGGCCT | pCCNE-R: TGATGGGGCTGCTCCGGCCT | ||
| − | |||
| + | (Product: 986) | ||
===References=== | ===References=== | ||
Revision as of 02:48, 11 October 2016
A cyclic promoter of Cyclin E from human genome
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 382
Illegal NotI site found at 497 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 529
Illegal XhoI site found at 1050 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 273
Illegal NgoMIV site found at 313
Illegal NgoMIV site found at 491 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:
pCCNE-F: CGTGTTTACATTCCACCCGCGCCA
pCCNE-R: TGATGGGGCTGCTCCGGCCT
(Product: 986)