Difference between revisions of "Part:BBa K1897003"
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===Usage and Biology===
 
===Usage and Biology===
  
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HasS (34.7 kDa) is the anti-sigma factor in the Has system signalling cascade. The Has system is a heme acquisition system originally from the ''Serratia marcescens''. HasS is responsible for binding to the cytoplasmic extracellular sigma factor, [https://parts.igem.org/Part:BBa_K1897005 HasI], inhibiting its activity. When the [https://parts.igem.org/Part:BBa_K1897001 holo-HasA hemophore] binds to the cell surface receptor [https://parts.igem.org/Part:BBa_K1897003 HasR], it triggers a conformational change in HasS. This then releases the HasI which binds to the [https://parts.igem.org/Part:BBa_K1897006 Has promoter]. This will allow for the expression of genes controlled by that promoter.  
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HasS (34.7 kDa) is the anti-sigma factor in the Has system signalling cascade. The Has system is a heme acquisition system originally from the ''Serratia marcescens''. HasS is responsible for binding to the cytoplasmic extra cytoplasmic function sigma factor, [https://parts.igem.org/Part:BBa_K1897005 HasI], inhibiting its activity. When the [https://parts.igem.org/Part:BBa_K1897001 holo-HasA hemophore] binds to the cell surface receptor [https://parts.igem.org/Part:BBa_K1897003 HasR], it triggers a conformational change in HasS. This then releases the HasI which allows for the expression of genes under the control of the [https://parts.igem.org/Part:BBa_K1897006 Has promoter].  
  
 
Characterisation of this part is done in tandem with the full has operon ([https://parts.igem.org/Part:BBa_K1897007 BBa_K1897007])
 
Characterisation of this part is done in tandem with the full has operon ([https://parts.igem.org/Part:BBa_K1897007 BBa_K1897007])
  
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===References===
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Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). ''Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition.'' Molecular microbiology, 53(4), 1267-1277.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1897003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1897003 SequenceAndFeatures</partinfo>

Latest revision as of 21:10, 10 October 2016


HasS antisigma factor coding sequence

Usage and Biology

HasS (34.7 kDa) is the anti-sigma factor in the Has system signalling cascade. The Has system is a heme acquisition system originally from the Serratia marcescens. HasS is responsible for binding to the cytoplasmic extra cytoplasmic function sigma factor, HasI, inhibiting its activity. When the holo-HasA hemophore binds to the cell surface receptor HasR, it triggers a conformational change in HasS. This then releases the HasI which allows for the expression of genes under the control of the Has promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
  • 1000
    COMPATIBLE WITH RFC[1000]