Difference between revisions of "Part:BBa K1897011:Design"

 
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===Design Notes===
 
===Design Notes===
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There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS <partinfo>BBa_K1897010</partinfo> is shifted to after the HA tag.
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There are two HA tags available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS <partinfo>BBa_K1897010</partinfo> is shifted to after the HA tags.
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===Source===
 
===Source===

Revision as of 18:55, 10 October 2016


Invasin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 2791
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 976
    Illegal AgeI site found at 2485
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are two HA tags available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS BBa_K1897010 is shifted to after the HA tags.

Source

Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [Genbank M17448.1] (see No part name specified with partinfo tag.. The promoter was synthesized based on the sequence obtained from BBa_R0062. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

References