Difference between revisions of "Part:BBa K2100005"
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<partinfo>BBa_K2100005 short</partinfo> | <partinfo>BBa_K2100005 short</partinfo> | ||
− | eYFP gene | + | eYFP is a fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. The promoter and gene are encoded within an expression vector backbone with an SV40 terminator site after the eYFP gene and an ampicillin resistant gene. |
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Revision as of 18:31, 10 October 2016
pENTR eYFP
eYFP is a fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. The promoter and gene are encoded within an expression vector backbone with an SV40 terminator site after the eYFP gene and an ampicillin resistant gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 222
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 222
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 222
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 222
Illegal AgeI site found at 5 - 1000COMPATIBLE WITH RFC[1000]