Difference between revisions of "Part:BBa K1933100"

 
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<partinfo>BBa_K1933100 short</partinfo>
 
<partinfo>BBa_K1933100 short</partinfo>
  
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CBDcex fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDcex) fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Westernblotting. For more information, please visit our <a ref=http://2016.igem.org/Team:Kyoto> Wiki page</a>.
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CBDcex fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDcex) fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Westernblotting. For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki].
  
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==Sequence and Features==
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1933100 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1933100 SequenceAndFeatures</partinfo>
  
  
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==Usage==
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===Functional Parameters===
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<partinfo>BBa_K1933100 parameters</partinfo>
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==Biology==
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==Characterization==
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==Reference==
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==Judging==

Revision as of 18:21, 10 October 2016


constitutive expression of CBDcex fused to INPNC with 6xHis tag

CBDcex fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDcex) fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Westernblotting. For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1012
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal NgoMIV site found at 465
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

Biology

Characterization

Reference

Judging