Difference between revisions of "Part:BBa K2100009"

Revision as of 18:07, 10 October 2016

pENTR pPRE4 The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.

When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 251
Illegal EcoRI site found at 278
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 19
Illegal BsaI.rc site found at 265

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2100009 short</partinfo>
+The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.
-description here.
+When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain.
 <!-- Add more about the biology of this part here