Difference between revisions of "Part:BBa K2100008"

 
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<partinfo>BBa_K2100008 short</partinfo>
 
<partinfo>BBa_K2100008 short</partinfo>
  
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description here.
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The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.
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When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain.  
  
 
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Revision as of 17:56, 10 October 2016


pENTR pPRE3

The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.

When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 225