Difference between revisions of "Part:BBa K2100003"

Revision as of 16:56, 10 October 2016

pENTR hEF1a

Enter description about hef1a promoter here. The pENTR hEF1a part is an entry vector containing the human elongation factor 1 alpha (hEF1a) promoter, acting as a constituitive mammalian promoter.

We have characterized the hEF1a promoter in all four cell lines we experimented on in the duration of our project using the following fluorescent tags: eYFP, mKate, and (tag?)BFP.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal BglII site found at 591
Illegal BamHI site found at 1197
Illegal XhoI site found at 990
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
Illegal NgoMIV site found at 725
Illegal AgeI site found at 103
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Enter description about hef1a promoter here.
+The pENTR hEF1a part is an entry vector containing the human elongation factor 1 alpha (hEF1a) promoter, acting as a constituitive mammalian promoter.
-In mamalian synthetic biology, the hEF1a promoter is used to constituitively express ...
+We have characterized the hEF1a promoter in all four cell lines we experimented on in the duration of our project using the following fluorescent tags: eYFP, mKate, and (tag?)BFP.
 <!-- Add more about the biology of this part here