Difference between revisions of "Part:BBa K1897008"
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This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015. | This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015. | ||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from <i>Vibrio fischeri</i>, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI (Shaldel and Baldwin, 1991). This also produces light due to the production of LuxCDABE (Shaldel and Baldwin, 1991). Transcription of operonL leads to the production of LuxR (Shaldel and Baldwin, 1991). In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon (Trott, 2000). | This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from <i>Vibrio fischeri</i>, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI (Shaldel and Baldwin, 1991). This also produces light due to the production of LuxCDABE (Shaldel and Baldwin, 1991). Transcription of operonL leads to the production of LuxR (Shaldel and Baldwin, 1991). In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon (Trott, 2000). | ||
This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062). | This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062). | ||
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Revision as of 15:44, 10 October 2016
LuxR
This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 50
- 1000COMPATIBLE WITH RFC[1000]