Difference between revisions of "Part:BBa K1897008:Design"
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===Source=== | ===Source=== | ||
| − | This part was made based on the genome of Vibrio fischeri. | + | This part was made based on the genome of <i>Vibrio fischeri</i>. |
===References=== | ===References=== | ||
Revision as of 15:40, 10 October 2016
LuxR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 50
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is one HA tag available for characterisation of the protein produced via western blot. Also, the terminator rrnBT1+BBa_B0012 was derived from BBa_B0015, with the first 8 base pairs removed.
Source
This part was made based on the genome of Vibrio fischeri.
References
Shadel, G. S., & Baldwin, T. O. (1991). The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of the luxR gene. Journal of bacteriology, 173(2), 568-574.
Trott, A. E. (2000). Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing (Doctoral dissertation, Virginia Polytechnic Institute and State University).