Difference between revisions of "Part:BBa K1898300:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The cDNA of CRYAA is ordered from Origene
+
The cDNA of CRYAA is ordered from OriGene
  
 
===References===
 
===References===

Revision as of 15:16, 10 October 2016


CRYAA, crystallin alpha A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.


Source

The cDNA of CRYAA is ordered from OriGene

References