Difference between revisions of "Part:BBa I716002:Design"

 
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
 +
{{BerkiGEM2007-Biobrick2.0Intro}}
 +
<pre>
 
Bbrick yfbE promoter
 
Bbrick yfbE promoter
 
[pcr] yfbE-F/R on E.coli MG1665 gen.    (437bp, BglII/XhoI)
 
[pcr] yfbE-F/R on E.coli MG1665 gen.    (437bp, BglII/XhoI)
Line 14: Line 17:
 
AY005.yfbE-F  cgacaAGATCTatgatatatttttttgacttc
 
AY005.yfbE-F  cgacaAGATCTatgatatatttttttgacttc
 
AY006.yfbE-R  cttatCTCGAGttaGGATCCcgagttcctccacgcccattg
 
AY006.yfbE-R  cttatCTCGAGttaGGATCCcgagttcctccacgcccattg
−
 
+
</pre>
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 19:36, 18 June 2007


yfbE iron promoter basic part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 25


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

Bbrick yfbE promoter
[pcr] yfbE-F/R on E.coli MG1665 gen.     (437bp, BglII/XhoI)
[sub] into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
[prod] pBca9145-yfbE (I716002)
---
AY005.yfbE-F   cgacaAGATCTatgatatatttttttgacttc
AY006.yfbE-R   cttatCTCGAGttaGGATCCcgagttcctccacgcccattg

Source

E. coli MG1665

References